Agilent microarray cgh protocol. 1 Briefly, four HL-60 targets and four normal targets were generated by DNaseI fragmentation of genomic DNA (10 μg) followed by biotin labeling using terminal transferase. Do the steps in “Step 1. It includes the SureScan Microarray Scanner, the G2565 Series Microarray Scanner accessories and SureScan Scanner accessories including backing slides, hybridization chamber, microarray hybridization oven, hybridization oven accessories, and stabilization & drying solution. The software finds and places microarray grids, rejects outlier pixels, accurately determines feature intensities and ratios, flags outlier pixels, and calculates statistical confidences. Agilent recommended CGH analysis settings (Default Analysis Method - CGH v2), CGH+SNP analysis Microarrays arrayed on 24-pack slides for Comparative Genomic Hybridization (CGH). This eliminates the use of a reference Jul 15, 2012 · Array-based comparative genomic hybridization is essentially Southern hybridization with multiple probes immobilized on a solid surface. The dual color microarrays contain 60-mer high quality probes to provide the sensitivity and specificity required for CNV association studies. CGH + (SNP) Microarray Analysis Platform. 2 Microarray Hybridization. The addition of the new SNP probes "allows for the detection of blocks of copy-neutral LOH or UPD down to 5 megabases without compromising protocol. ” It Features. 3-24. This protocol is specifically developed and optimized to amplify and enzymatically label DNA from single cells, and to hybridize to 8×60K CGH microarrays. ” It In this step of the wizard, you select the species that best represents the content of the microarray you want to create. 0 mg/mL. Microarray Washing 7 Step 6. This protocol is specifically developed and optimized to enzymatically label DNA from blood, cells or frozen tissues. There's lots of probe on each spot, so they Abstract. protocol. Feature Extraction identifi es the Abstract. Design ID. Formatted as 4x44 K high-definition microarrays on barcoded, 1' x 3' (25 mm x 75 mm) glass slides. 1 Clone Selection. 0). Exon-by-exon coverage of cytogenetically relevant genes. Agilent Oligo aCGH is the solution • Provides higher resolution and accurate measurement • Great flexibility in probe design and selection that delivers superior performance which meets the demands of your workflow • Integration of gene expression information for better and faster discovery research via novel data Dec 2, 2016 · A number of assays have been developed for detection of CNVs in the human genomes, including fluorescence in situ hybridization (FISH), array-based comparative genomic hybridization (aCGH), PCR-based assays, and next-generation sequencing [ 1 – 3, 13 ]. Our software suite includes Feature Extraction Learn about our selection of CGH & CGH+SNP Microarray Scanners & Equipment for your research and diagnostic workflows. WGA product generated from intact HT29 DNA (A) and fragmented HT29 DNA (B) revealed identical known deletion across the 8p arm (horizontal shift to left of zero line), amplification along the 8q arm in the 8q23. These special databases contain probes that cover the genomes of several species at extremely high densities. Discover Agilent’s CGH microarray portfolio, an extensive selection of dedicated microarrays for your cytogenetic workflow. Agilent 60-mer SurePrint Technology. Each slide contains four identical arrays consisting of ~43,000 in situsynthesized 60-mer Agilent Feature Extraction (FE) Version 9. PreventionGenetics. This protocol is The GenetiSure Pre-Screen Amp and Labeling Kit is used with the GenetiSure Pre-Screen microarray for sample preparation, allowing sample-to-results in 8 hours. 5 hours. The Agilent HT microarrays for aCGH are available in two different formats: 16-pack microarray slides with 16 microarrays/slide and 24-pack microarray slides with 24 microarrays/slide. We provide whole genome CMA at variable resolutions, down to exon level, CGH+SNP arrays to analyze CN and LOH in a single experiment and application specific designs, including pre- post-natal, PGS and Cancer specific microarrays. 2. Microarray Hybridization 6 Step 5. Discover our selection of CGH + SNP Microarray software for your workflows and clinical research needs. 22. 43,803 porcine probes represented. Microarray Scanning and Analysis 8 This Quick Reference Guide is an abbreviated version of publication G4410-90010, “Oligonucleotide Array-Based CGH for Genomic DNA Analysis - Enzymatic Labeling, For Blood, Cells, or Tissues (with a High Throughput option) Protocol. 3. We have optimized several different protocols and introduce a high-throughput approach to perform a cost-effective, fast, high-throughput and high-quality CMA. This array consists of ~43,000 in situ synthesized 60-mer oligonucleotide probes that span coding and noncoding sequences with an average spatial resolution of ~35 kb. The system takes advantage of Agilent’s dual-color CGH technology and uses a specific protocol that allows sample vs sample hybridization and analysis against both male and female reference samples for optimal Agilent CGH microarrays are widely recognized as the industry standard for genome-wide chromosomal analysis. We show that comparative genomic hybridization microarrays fabricated with maskless array synthesizer technology can be used up to four times with the application of 1,3-dimethylurea as an array-stripping agent. CGH Protocol Optimization . This allows for the simultaneous, high-resolution detection of copy number and copy-neutral variations and eliminates the need to run two separate Comparative genomic hybridization analyses have contributed greatly to our understanding of bacterial evolution, population genetics, and pathogenesis. Specification. Target cDNA (for gene expression) or genomic DNA (for CGH) is often radiolabelled using 32P or 33P, so very sensitive detection is possible. 2With clean, powder-free gloved fingers, remove the gasket slide from its package. See What is a High Density (HD) Search. If you are a new user, refer to publication G4410-90010, Oligonucleotide Array-Based CGH for Genomic DNA Analysis - Enzymatic Labeling, For Blood, Cells, or Tissues (with a High Throughput option) Protocol, which is the full-length The Agilent Pre/Postnatal and cancer research CGH & CGH+SNP Microarrays are dual-color microarrays containing 60-mer oligonucleotides designed and optimized by Baylor College of Medicine experts to target regions relevant to these areas of research. 3. Agilent’s Feature Extraction software (version 9. With easy-to-use catalog products for a variety of CGH applications and the industry’s most affordable and flexible custom content program, Agilent’s CGH solution provides capabilities that can adapt to the changing needs of your laboratory and your research program. 7. It is validated for processing of Agilent’s aCGH, ChIP-on-Chip and DNA methylation microarrays. Consistent feature (spot) quality from microarray-to-microarray Comparative genomic hybridization. Lab Operations Management Agilent recommended Feature Extraction protocol for CGH microarrays (CytoCGH_0105_May11) and QC metric set for CGH microarrays (CytoCGH_QCMT_May11) are provided as default. The GenetiSure Cancer Research CGH+SNP microarray is designed to target cancer regions of the genome sourced from the COSMIC and CGC databases. 650 mL of Cot-1 Human DNA at a concentration of 1. Feature Extraction Software - Revision Table. Comparative genomic hybridization ( CGH) is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. Although probes are slightly more closely spaced on the 4x180K array compared to the 8x60K microarrays, the effective biological resolution is the same for both microarray formats. The total time from amplification to data analysis can be as short as 6. The Agilent Human Genome CGH Microarrays (44B) were used. Our selection includes the Cytogenomics Software which caters to the needs of cytogeneticists for analysis and triage of their CGH and CGH+SNP data from pre- post-natal, PGS and cancer samples. Scanner • Agilent CytoGenomics Software Lab Management Services; Maintenance & Repair. The QIAGEN REPLI-g MDA whole genome amplification technology used in this kit enables highly uniform amplification across the entire genome, with minimal locus bias during amplification. 23 region (horizontal shift to right of zero line), and a focal deletion in 8q23 In this chapter, we present details requisite to successful application of the array-CGH technique on spotted “home-made” HEEBO microarrays, including ( 1) preparation, labeling, and hybridization of genomic DNA, ( 2) processing of microarrays, and ( 3) post-hybridization protocols, including microarray washing, imaging, and data analysis. The supplies and accessories come with features that ensure optimal performance. HD Searches are searches of the Agilent HD-CGH or HD-ChIP databases. 1. The CGH probes are gene- and exon-biased The Agilent miRNA complete labelling and hybridization kit provides a unique way to analyze your samples. 5 or 12. Widest dynamic range of over 5 logs ensures detection of low and high expressors to accurately represent the range of expression levels. 4 kb overall median probe spacing. Learn about Agilent’s industry leading CGH + SNP Microarray platform for your workflows and clinical research needs. 31, 2005- Products Enable Researchers to Conduct Genome-Wide Analyses of Chromosomal Changes in Cancer Cells Agilent Technologies Inc. Agilent’s High-Definition Comparative Genomic Hybridization (HD-CGH) database provides users the flexibility to create custom microarray designs for analysis of genome regions of interest to them at the resolution of their choosing. The first step in array-CGH is the design or choice of the microarray to be used for interrogating test genomes. Labeling, Hybridization and Washing. 5 includes the following new and changed functions: †Ability to extract Agilent micro RNA (miRNA) microarrays. A single tube contains 0. Jan 1, 2016 · Comparative genomic hybridization (CGH) also known as chromosomal microarray analysis (CMA) is the best available tool to detect copy number variations in chromosomal make up. ” It Model Organism CGH Microarrays. The array provides comprehensive coverage of the genome, including coding and non-coding regions with emphasis on known genes. Here, we describe a robust protocol for microarray-based comparison of genome content, which could be applied to any bacterial species of interest. the process, and reduces sample mix-up in high-throughput DNA microarrays. GenetiSure Cyto CGH and CGH+SNP Microarrays are a set of three different microarray designs with content intended for the detection of copy number variations (CNV) only or CNV and copy-neutral loss of heterozygosity (cnLOH) in constitutional DNA samples extracted from blood, saliva, amniotic fluid or CVS. Array-Based CGH for Genomic DNA Analysis - Enzymatic Labeling 3 In This Guide… This guide describes the Agilent recommended operational procedures to analyze DNA copy number variations using Agilent 60-mer oligonucleotide microarrays for array-based comparative genomic hybridization (aCGH) analysis. 0,’ was used for microarray hybridization. Agilent-CGHblock (a component of the Oligo aCGH Labeling Kit for FFPE Samples) was added and samples were hybridized to the Agilent Human Genome CGH 4 x 44K Microarray (P/N G4426B, AMADID 014950). Agilent recommends including the following types of probe sets when generating Step 4. The product should be stored at 4 °C to ensure integrity is preserved. Mouse Gene Expression Microarrays provide comprehensive coverage of genes and transcripts using the latest annotation databases. D. To remove gasket slide from its packaging. Do the steps in “Step 2. Array-CGH involves the comparison of a test to a reference genome using a microarray composed of target sequences with known chromosomal coordinates. It provides for better distribution of the sample over the active area, and helps prevent the occurrence of gradients in the signals. Feature. 5 KB in Refseq genes) Manufacturing. The GenetiSure Dx Postnatal Assay uses Agilent's proprietary aCGH for copy-number and LOH data, enabling cytogeneticists to accurately detect genetic anomalies associated with developmental delay, intellectual disability, congenital anomalies, and dysmorphic features. 5 Mb, ensures that important aberrations are not missed. Refer to the Agilent Oligonucleotide Array-Based CGH for Genomic DNA Analysis – Enzymatic Labeling for Blood, Cells, or Tissues (with a High Throughput option) Protocol (p/n G4410-90010) for this step. The Agilent Mouse and Rat CGH Microarray is a dual color array containing 60-mer high quality probes. Find out more. Loss of heterozygosity (LOH) detection, with a resolution validated to 2. The protocol uses the Agilent SurePrint G3 Human CGH 8x60K or 4x180K Microarrays, and a Multiple Displacement amplification (MDA) method. For processing DNA isolated from blood, cell culture, or frozen tissues, follow the Agilent Learn about our selection of CGH & CGH+SNP Microarray Supplies for your clinical research, CGH + SNP experiments and workflow needs. Cot1 DNA is validated for use with Agilent Methylation and Chip-on-Chip microarrays. 500 ng genomic DNA or 3 µg amplified genomic DNA. Feature Extraction software delivers the maximum amount of high-quality data from each and every microarray run. SurePrint G3 Human Genome CGH+SNP Bundle, 2x400K. The full-length The Agilent Oligo aCGH Prehybridization Buffer is used to wet and condition arrays for hybridization on a Tecan HS 400 Pro or HS 4800 Pro with compatible hybridization chambers. Mouse Genome CGH Microarray, 2x105K 1 slide / 2 samples G4425B-014699 Rat Genome CGH Microarray, 1x244K 1 slide / 1 sample G4423B-015223 Rat Genome CGH Microarray, 2x105K 1 slide / 2 samples G4425B-015235 SurePrint G3 CGH Rat Microarray, 1x1M 1 slide / 1 sample G4824A-027065 SurePrint G3 Rat CGH Microarray Kit, 4x180K 3 slides / 12 samples CGH & CGH+SNP Microarray Buffers. For processing FFPE samples, follow the Agilent Oligonucleotide Array-Based CGH for Genomic DNA Analysis (ULS Labeling RUO. (CGH and Expression application types only) In Select Array Type, select one of these options: Standard – For the CGH application type, this option creates a standard CGH microarray design. Now, the platform has been extended to support hematologic cancer research. This Quick Reference Guide is intended for experienced users who are already familiar with processing Agilent Oligo CGH Microarrays arrayed on 8-pack slides. Table of Contents. Iman Kishawi, Ph. For Research Use Only. Step 4. Agilent's feature extraction software automatically reads and processes up to 100 raw microarray image files. Briefly vortex the 2× Reaction Mix (included in the BioPrime Total for Agilent aCGH labeling kit) and centrifuge to collect the contents of the tubes. Agilent provides a complete platform including microarrays, reagents, hardware and software. Cytogenetically relevant microdeletion, microduplication, pericentromeric and subtelomeric regions. The kit is validated for use with Agilent microarrays. GenetiSure Cyto CGH & CGH+SNP Microarrays. If you are a new user, refer to publication G4132-90000, High-Throughput aCGH Analysis using Agilent HT Microarrays- Enzymatic Labeling of gDNA with the SureTag HT Kit Protocol, which is the full-length version of this Quick Reference Guide. Materials and Methods Agilent offers two different protocols for use with CGH microarrays allowing users to directly label the genomic DNA (direct) or first phi-29 The protocol for CGH analysis using gene expression microarrays has been described elsewhere. The molecules are directly labelled, with no need for enrichment at the beginning or purification after labelling. Adding the spike-ins to samples ensures the identity of the sample throughout. Comparative genomic hybridization (CGH) is used to study the chromosomal changes that 8 Agilent Microarray Hybridization Chamber User Guide. QC Chart Tool and Metric Sets. The current SurePrint G3 Human Comparative Genomic Hybridization (CGH) platform has been extended to include a set of Single Nucleotide Polymorphism (SNP) probes on the same microarray. Prepare the 10× Blocking Agent” in the chapter “Microarray Processing”. Refer to the Agilent Oligonucleotide Array-Based CGH for Genomic DNA Analysis (Enzymatic Labeling for Blood, Cells, or Tissues) Protocol (publication G4410-90010). In principle, this method can be applied to many other molecular genetic. CGH Analytics ideograms illustrating data from Agilent Human Genome CGH 44B microarrays. 1 Before You Begin This chapter contains information (such as procedural notes, safety information, microarray protocols, and more importantly, the ability of our oligo aCGH platform to detect chromosomal alterations throughout the human genome with high sensitivity and precision. oligonucleotide microarrays for array-based comparative genomic hybridization (aCGH) analysis. CGH Microarray Solutions for Genome-Wide Genetic Analysis. Enriched probe coverage. Add 25 μL of Alexa 5 Fluor 2× Reaction Mix to each tumor sample and 25 μL of Alexa 3 Fluor 2× Reaction Mix to each reference sample. The manufacturer’s methodology supplied with the GenomePlex Complete WGA Kit (Sigma) was used for DNA amplification, and the Agilent protocol G4410-90010, ‘Agilent Oligonucleotide Array-Based CGH for Genomic DNA Analysis—Version 6. Feature: Specification: Product Number: G4436A: All Agilent microarrays use a common Anniek De Witte, CGH product manager in Agilent's Life Sciences Group, told last week that the firm's SurePrint G3 CGH microarrays will soon be extended to include a set of SNP probes on the same microarray. Platform • ag CompleteSureT DNA Labeling Kit • Custom CGH+SNP Microarrays • Baylor College of Medicine Chromosomal Microarrays • Oligo aCGH Hybridization Kits, Buffers, and Processing Components • SureScan Microarray. These microarrays are designed with genome-wide backbone probes and The GenetiSure Pre-Screen microarray screens for DNA copy number variations and chromosomal aneuploidies in human cell samples collected from embryo biopsies and single cell samples. n i g e B u o Y e r o f e1B introduction of DNA spike-ins to human DNA samples before wet lab handling. The SurePrint G3 Mouse Gene Expression v2 Microarray features complete coverage of establish RefSeq coding transcripts (NM) from the latest build and updated long non-coding RNA (lncRNA) content to ensure Mar 10, 2006 · Advantages: Tend to be cheaper to buy and use than microarrays, as each array can be 'stripped' and reused 2-3 times (unlike microarrays, which are single-use). Place the tubes on ice. Superior results and a complete workflow for Agilent microarray-based applications such as CGH, CGH+SNP, CNV, ChIP-on-chip and DNA methylation. There are two common strategies: (i) the design or the selection of one microarray covering the whole genome in order to screen for every deletion or duplication in a given test genome compared to a reference DNA; (ii) the construction and use of one Seamless CGH Diagnostic Testing. Microarray Hybridization Oven Discover Agilent’s CGH microarray portfolio, an extensive selection of dedicated microarrays for your cytogenetic workflow. Three micrograms of genomic DNA was digested with AluI (50 units) and RsaI (50 The Agilent SurePrint G3 CGH+SNP microarray platform enables high-resolution CGH analysis, making it a powerful tool for detecting genome-wide copy number and copy-neutral genetic aberrations. The high quality CGH hybridization solution provides reliable data for many different experiments. For the Expression application Jan. The full-length conducted according to the aCGH protocol above. †Updated FE protocols for all formats. SurePrint G3 Bovine Genome CGH Microarray Kit, 4x180k : SurePrint G3 Canine Genome CGH Microarray Kit, 4x180K : Chicken Genome CGH Microarray Kit, 1x244K : SurePrint G3 Chimpanzee Genome CGH Microarray Kit, 4x180K : SurePrint G3 Rice Genome CGH Microarray Kit, 4x180K : SurePrint G3 Rhesus Macaque Genome CGH Microarray Kit, 4x180K : Agilent . The total number of reactions per array format depends on the format of the array used for the experiment. The Agilent SurePrint G3 ISCA v2 CGH and CGH+SNP microarrays are dual-color chromosomal microarrays containing 60-mer high quality probes. The Most Trusted Cytogenetics Research Solutions. Jan 1, 2009 · 3. † Updated protocols can process images of Agilent microarrays scanned on Roche NimbleGen MS 200 scanners, Molecular Devices GenePix scanners, select Innopsys InnoScan scanners, and select Tecan scanners; see the scanner compatibility matrix for more information † New QC metric (LogRatioImbalance) to flag CGH microarrays with a CGH array design guidance. Service Plans, On Demand Repair, Preventive Maintenance, and Service Center Repair. 8. The aim of this technique is to quickly and efficiently compare two Jan 10, 2024 · SurePrint G3 Human CGH Microarray Kit, 4x180K (Agilent) containing glass slides each formatted with four SurePrint G3 180K arrays. G5921A Option 2. Agilent 60-mer oligonucleotide microarrays for array-based comparative genomic hybridization (aCGH) analysis. The two probe DNAs are co-hybridized on a microarray in the Array Comparative Genomic Hybridization (CGH) • Microarray-based comparative genomic hybridization (array CGH) is a powerful method for the genome wide detection of chromosome copy number changes at a higher resolution level than conventional chromosome-based CGH • Based on the co-hybridisation of differentially labelled test and Note that this protocol does not support analysis of CGH+SNP microarrays. Our portfolio of Array CGH experiment supplies includes backing slides, hybridization chamber, microarray hybridization oven Dec 21, 2015 · The catalog 2x400K SurePrint G3 CGH+SNP array contains approximately 300,000 CGH probes and 120,000 SNP probes. Jan 1, 2013 · 1. Microarrays arrayed on 16-pack slides for Comparative Genomic Hybridization (CGH). Our range of high quality reagents include Stabilization & Drying Solution for ozone control, CGH Hybridization and Wash Buffer for microarray processing. 0 to 12. These are high-resolution tools for genome-wide DNA copy number variation profiling, and +SNP option to capture copy-neutral loss of heterozygosity (cnLOH), without amplification or complexity reduction. 2kb overall median probe spacing (4. Sufficient reagents are included for 50 labeling reactions (or 100 reactions for the 8-pack system). Genotypes are measured using two SNP probes per SNP, providing the highest call rate and accuracy per SNP, and resulting in ~5–10 Mb resolution for LOH/UPD detection across the entire genome. When used in conjunction with a high-density Agilent microarray and an Agilent Microarray Scanner, it extends the dynamic range to accurately measure both very high- and very low-intensity features. We provide different formats to accommodate resolution and throughput. All the buffers have been validated to provide optimal Agilent’s CGH Wash Buffer Kit is used for removing non-hybridized or partially hybridized labeled sample DNA from the array's surface to minimize signal noise. Jan 1, 2011 · This is followed by the addition of 130 μl of Agilent CGH Block to the hybridization cocktail, mixing and avoiding bubbles in the mix. Microarrays were scanned using an Agilent DNA Microarray Scanner (Agilent P/N G2565BA) with Agilent Scanner Control Software (version 7. The arrays provide different formats and resolutions to match research needs. Feature Extraction Protocols - Download. 2. You use this wizard to create a microarray design that uses probes from an HD Probe Search. From custom and catalog CGH+SNP to gene expression microarrays and more, Agilent supports you in a variety of applications including prenatal, postnatal and tumor sample analysis. Agilent CGH 13. The aCGH has many advantages over the conventional cytogenetic techniques, such as genome The Agilent Human Genome CGH Microarrays (44B) were used. 1With tweezers, carefully lift up the corner of the clear plastic covering and slowly pull back the protective sheath. Hybridization Chamber Kit – SureHyb enabled, Stainless (Agilent) containing: stainless steel chamber (top and base), thumb clamp and screw, plastic tweezers, user’s guide. This protocol is specifically developed and optimized to use the Bravo Automated Liquid Handling Platform for the labeling (ULS and enzymatic), purification and pre-hybridization steps. General Refer to the Agilent Oligonucleotide Array-Based CGH for Genomic DNA Analysis – Enzymatic Labeling for Blood, Cells, or Tissues (with a High Throughput option) Protocol (p/n G4410-90010) for this step. CGH Microarray Solutions. Migration Utility User Guide for Feature Extraction 11. The eight targets were individually hybridized to eight GeneChip® Human Genome Discover Agilent’s CGH microarray portfolio, an extensive selection of dedicated microarrays for your cytogenetic workflow. Apr 21, 2012 · Human Genome CGH Microarray 44B And V2. The GenetiSure Dx Postnatal solution Validated on Agilent SureScan and Innopysy (Innoscan 710 ) scanners Validated on Agilent scanners Technical description Table 1. The Chromosomal Microarray Workflow • Bravo Automated Liquid Handling. The protocol has been optimized to be fast and limit processing bias, providing optimal sensitivity, specificity Table presents specifications for the Rat Genome CGH Microarray Kit 105A. With almost 20 years’ experience and thousands of publications, Agilent’s CGH platform is widely adopted by cytogenetics labs running pre- and post-natal, preimplantation and Learn how Agilent Human CNV Microarrays provide high-resolution optimized probe design to enable genome-wide CNV identification and characterization. Chemical Analysis, Life Sciences, and Diagnostics | Agilent Discover Agilent’s CGH microarray portfolio, an extensive selection of dedicated microarrays for your cytogenetic workflow. Algorithms developed for the concurrent Oligonucleotide Microarrays. The design options and aCGH protocol for the HT microarrays differ from those for the standard Agilent aCGH microarrays of smaller pack size. Feature Extraction Software. Agilent’s microarray analysis portfolio offers you unprecedented flexibility with superior quality. Two unknown samples are hybridized on the same array. 9. Agilent 60-mer SurePrint technology. The Agilent CGH Microarray Platform – powered by SurePrint and SureScan technologies for over 10 years – sits at the center of an integrated research platform designed for highly-sensitive, highly-scalable, and highly-reproducible profiling with low DNA input amounts. Not for use in diagnostic procedures. The test and reference DNA samples are used as templates to generate two probe DNAs labeled with distinct fluorescent dyes. (NYSE:A) today introduced its Human Genome CGH Microarray and CGH Analytics software to help cancer biologists and cytogeneticists study genomic alterations in cancer. Effective for genomic DNA isolated from tissues, cells, blood and FFPE samples, this non-enzymatic method directly labels genomic DNA with fluorescent dyes, thereby The Agilent CGH Microarray Platform – powered by SurePrint and SureScan technologies for over 10 years – sits at the center of an integrated research platform designed for highly-sensitive, highly-scalable, and highly-reproducible profiling with DNA of different types and quality. While the GenetiSure postnatal research array allows simultaneous The Agilent Hybridization Kit provides optimized hybridization kinetics, enhanced signal intensity and maximum noise reduction. Utilizing Kreatech's Universal Linkage System (ULS™), Agilent's Genomic DNA ULS Labeling Kit efficiently generates Cy-labeled genomic targets for use with Agilent CGH microarrays. An Agilent microarray slide has two sides. Discover our collection of CGH & CGH+SNP Microarray Buffers for your experiments and workflows. This eliminates the use of a reference for 3 minutes and incubated at 37°C for 30 minutes. 5) was used to extract data from raw microarray image files in preparation for analysis. Additional reagents are required to prepare the hybridization solution, such as Cot-1 DNA, DNase/RNase-Free distilled Agilent custom microarrays allows users to design and print custom CGH and CGH+SNP microarrays, enabling you to create arrays that can detect copy number changes and copy neutral aberrations, such as loss of heterozygosity (LOH) and uniparental disomy (UPD), on the same array. Manufacturing. †Improved gridding and workflow for multiplex (formerly known as multipack) microarrays. Finally, the tubes are centrifuged for 10 s to collect the cocktail at the bottom. Agilent Product Number. Figure 4. ts jh ly iu xe vl rw sp ht ml